Preparative liquid chromatography system Nexera™ Prep

HPLCUFLCfor research and development

Overview
The Nexera Prep is a preparative purification liquid chromatograph designed to provide optimal solutions for laboratory preparative and purification workflows. It supports scale-up from analytical to preparative separations and integrates automation for efficient fraction collection and high-purity target recovery.

Key benefits

• Easy optimization of preparative parameters and scale up, with fractionation simulation to rapidly configure collection logic and column line-up for analytical-to-preparative transitions.
• Time- and energy-saving automation of the purification workflow, including automated desalting and fraction collection for purification of target components at high purity.
• Expandable architecture to suit sample and fraction counts and volumes, with a wide range of recovery scale and analytical detection options to meet diverse application needs.

Features

• Streamlined condition development: simplifies scaling up from analytical methods to preparative conditions, reducing manual setup time.
• Preparative purification at high concentration and purity: UFPLC (Ultra Fast Preparative and Purification Liquid Chromatograph) reduces cost and labor while enabling high-yield recovery of targets and impurities for direct impurity analysis.
• Support for non-UV-absorbing targets: LH-40 and FRC-40 enable signal-based logic and collection across up to four signal channels; detection options include LCMS, RID and ELSD.
• Efficient workflow from setup to processing: Open Solution software centralizes operations and supports multi-user environments.
• Preparative recycling mode: repeated column cycling improves separation and recovery of targets from coeluting species without requiring longer or multiple columns.
• System expandability: selectable solvent delivery units and fraction collectors, multiple sample introduction and reinjection options, and Shim-pack Scepter columns for scalable phase choices.

Applications / Application notes
Documents | Creation Date
Automated Preparative Purification Using Nexera UFPLC — High Concentration Collection via an Online Trap Column | 2026-05-07
Investigation of Separation Conditions and Prep Purification of GLP-1 Receptor Agonist Semaglutide | 2025-11-25
Separation of Styrene Oligomers Using Recycle Fractionation System | 2025-11-18

Technical Documents
Documents | Creation Date
Optimizing Preparative Workflow with the LH-40 Liquid Handler | 2022-01-17
Preparative HPLC Primer | 2021-07-08

Manuals
Documents | Creation Date
Nexera Prep System Guide | 2025-11-10
Safety Guideline - Nexera Prep Series | 2025-05-15
LH-40 Instruction Manual - Liquid Handler | 2025-04-23

Trademarks
Nexera, Open Solution, Shim-pack Scepter, LabSolutions and Purification Solution are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.

Technical specifications

• System type: Preparative purification liquid chromatograph (Preparative HPLC / UFPLC).
• Scale-up capability: column line-up and Shim-pack Scepter columns for scalability from analytical to preparative separations.
• Automation: automated purification workflow including fractionation simulation, automated desalting and fraction collection.
• Detection options: supports LCMS, Refractive Index Detector (RID), Evaporative Light Scattering Detector (ELSD) and multi-channel signal-based logic (up to 4 channels with LH-40 and FRC-40).
• Liquid handling: LH-40 liquid handler integration for improved preparative workflow and high-concentration collection (online trap column capability described in application notes).
• Recycling fractionation: preparative recycling mode for enhanced separation of coeluting species.
• Software: Open Solution software for streamlined operations and multi-user support.
• Expandability: selectable solvent delivery units and fraction collectors; sample introduction and reinjection options for varied recovery volumes.