Solution reagent kit GEXSCOPE®

enzymepathologyfor RNA library preparation

Single-cell RNA technologies have rarely been adapted to microorganisms because of challenges related to their size, lower RNA content within a single cell and strict lysis requirements. Singleron developed the first commercially available product to enable high throughput single-cell RNA-Seq from yeast models. This novel technology enables researchers, pathologists, food industries and fermentation businesses to study stochastical variations of gene expression in yeast population through single-cell sequencing with reliable and standardized results. GEXSCOPE® Microbial Single Cell RNA Library Kit (Yeast) is ideally suited to study heterogeneity within a microbial yeast population. This kit offers the complete solution to generate single-cell sequencing ready libraries within one day starting from fixed or fresh cells. The kit includes Singleron’s proprietary SCOPE™ chips for cell partitioning and AccuraCell® beads for unique cell barcoding, an optimized lysis buffer for the digestion of the yeast cell wall, as well as enzymes, buffers, and indices for cDNA and sequencing library generation. The kit was optimized to capture over 30 000 single cells per chip and is compatible with a fully automated cell parsing instrument. Highlights • Easy to use: All-in-one solution developed and tested for yeast studies • High throughput: Sequence 500-30.000 yeast single-cells per chip • Fast: From sample to sequencing library within one working day • Wide area of applications: From basic yeast research to tailoring yeast cells for industry