Ultrapure dUTP (2´-Deoxyuridine, 5´-Triphosphate) supplied as sodium salt in purified water (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Avoid carryover contamination between PCRs to eliminate a source of false positives.
-20°C for 24 months
Greater than 99% purity confirmed by HPLC
The purity of SMOBIO’s dNTP remained greater than 99% after one week storage at 25°C.
SMOBIO’s dNTPs are enzymatically synthesized and undergo stringent purification steps to
eliminate impurities and PCR-specific inhibitors such as modified nucleotides, tetraphosphates and
pyrophosphates commonly observed in other commercially available dNTP products. SMOBIO
offers greater than 99% purity of dNTPs to yield optimal results.
Impurity or residual PCR inhibitors in dNTP mix suppress DNA amplification.
(A) High fidelity DNA polymerase, which is highly sensitive to PCR inhibitors, was completely failed in amplification using dNTP mix from other supplier.