Loading buffer reagent PM2700

for microbiologyfor molecular biologyprotein

The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Features Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil. Two reference bands — 75 kDa (red) and 25 kDa (green) Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Quality Control Under suggested conditions, PM2700 ExcelBand™ 3-color Broad Range Protein Marker resolves 13 major bands in SDS-PAGE (Bis-Tris gel, MES buffer) and after Western blotting to nitrocellulose membrane. Specification Product Size 2 x 250 μl MW Range 5 – 245 kDa Band Number 13 Band Color Red/Green/Blue Markered Bands 25, 75 kDa