Solution reagent kit FIREScript® RT cDNA synthesis MIX

reverse transcriptaseRNase inhibitorfor microbiology

Convenient cDNA synthesis mix with fast and robust FIREScript® Reverse Transcriptase. Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures High specificity and yield. Wide reaction temperature from 37°C to 60°C. Fast 15 min reaction time. A convenient 3-vial kit with all reaction components. RNase inhibitor and water included. Reaction set-up and shipment without ice. Description FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR). Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme mix with all reagents included Enzyme: FIRESript® Reverse Transcriptase Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene. Reagents FIREScript® Enzyme Mix (FIREScript® RT and RiboGrip RNase inhibitor) 10x RT Reaction Premix without primers RT reaction buffer with DTT and dNTPs Water, nuclease free Some applications of this product may require a license which is not provided by the purchase of this product.