Antiserum reagent OK O

serumantibodyantigen

OK O antisera are suitable for screening for surface antigens on live cultures, and are particularly suited to test for known EPEC and/or VTEC serotypes. A positive agglutination test is not indicative of the presence of relevant virulence genes. Positive isolates should therefore be investigated further for virulence factors, e.g. using PCR or H serotyping. Product description OK O antisera are polyclonal antisera produced by immunising rabbits with whole formalin-killed cells. Serum therefore contains antibodies against O, K, H and other protein antigens found on the surface of the cell. For this reason OK O sera are intended only for screening and must always be confirmed with O serotyping. OK O antisera are supplied in dropper bottles of 3 mL ready-to-use antiserum, sufficient for at least 150 reactions. We offer the full range of antisera for E. coli serotyping High quality All absorbed free of cross-reactions We offer experienced technical How to use OK O antisera are used for slide agglutination with live cultures, and the reaction can be read after 5-10 seconds. Pool OK O antisera are absorbed in OK O pool 1-3. OK O single antisera are absorbed within the EPEC/VTEC O groups contained in the pools. The result of the test can be attributed to the presence of several different surface structures, and so false positive and sometimes false negative reactions can occur. It is therefore very important to have the O group confirmed using agglutination with a specific O antiserum.