Recombinant RNase Inhibitor ver.2.0 is an oxidation-resistant RNase inhibitor that is highly stable in enzymatic reactions requiring low DTT concentrations, such as in vitro transcription, in vitro translation, or RT-PCR. Derived from porcine liver, Recombinant RNase Inhibitor ver.2.0 displays greatly enhanced oxidation resistance due to a mutation in a cysteine residue that is very sensitive to oxidation, which prevents degradation and inactivation of the enzyme under oxidizing reaction conditions.
The enzyme forms a 1:1 complex specifically with RNase A to inhibit RNase activity; however, it is not effective against RNase H activity. The inhibitory reaction is reversible, and the inhibitor can be irreversibly inactivated to restore ribonuclease activity by dissociating the complex with urea or a sulfhydryl reagent. Moreover, unlike other non-protein competitive inhibitors (e.g., nucleotides and inorganic phosphates), it can easily be removed from the reaction system by phenol extraction.
Recombinant RNase Inhibitor ver.2.0 contains a mutation in a cysteine residue that is very sensitive to oxidation, making it highly stable in enzymatic reactions requiring low DTT concentrations. It inhibits RNase A activity through the formation of a 1:1 complex in a reversible reaction. It is not effective against RNase H activity.
Efficient RNAse A inhibitor
Highly stable against oxidation
Ideal for applications requiring low DTT concentration, such as in vitro transcription, in vitro translation, or RT-PCR
Suitable for long-term storage of samples
In vitro transcription/translation (1 U/µl reaction)*