Veterinary test kit AD0480

veterinaryenzyme immunoassay

Description: • The kit is standardized according to the OIE International Reference Standard Serum (Community reference serum ADV1). • The microtitre plate is coated with inactivated and purified suid herpesvirus 1 antigen. • The kit enables 480 screening tests, including controls. • The whole kit may be used sequentially for smaller batches of samples. • Microtitre wells are coated with the viral antigens. • ELISA is more sensitive than the virus neutralisation test (VNT) using the 100 TCID50. • Total assay time: approximately 1,5 h. • Tested at the Veterinary Research Institute, Brno, Czech Republic and State Veterinary Institutes in the Czech Republic. • Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic. • The kit is manufactured in accordance with Good Manufacturing Practice (GMP). • Shelf life: 24 months. Application: • Screening of Aujeszky's disease occurrence in swine herds. • Diagnosis of the infection. • Checking of vaccination effectiveness. • Control of transported and quarantined animals (export, import). • Determination of antibodies in samples of imported unprocessed meat. Brief assay procedure: 1. Dilute samples and pipette them into the wells (1:20). 2. Incubate for 30 min at 37 °C. 3. Aspirate and wash the wells 4 times. 4. Add Conjugate. 5. Incubate for 30 min at 37 °C. 6. Aspirate and wash the wells 4 times. 7. Add substrate (TMB-Complete). 8. Incubate for 15 min at room temperature.