Proteinase K reagent GE201-01
proteasefor DNA extractionfor RNA extraction

proteinase K reagent
proteinase K reagent
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Characteristics

Type
protease, proteinase K
Applications
for DNA extraction, for RNA extraction
Tested parameter
genomic DNA
Storage temperature

Min.: 15 °C
(59 °F)

Max.: 25 °C
(77 °F)

-20 °C
(-4 °F)

Description

Proteinase K is a nonspecific serine protease that will hydrolyze a variety of peptide bands. Proteinase K is active in a broad range of temperature and buffers. It cannot be inactivated by metal ions, chelating agents (e.g., EDTA), or detergents such as SDS. • Active in a wide range of buffers and pH value. • Incubation temperature: 55-65℃; optimal temperature: 58℃. • Incubation time: 15 minutes to 48 hours; optimal incubation time: 2 hours. Applications • Preparation of DNA and RNA. • Inactivation of RNase, DNase and enzymes. • Isolation of genomic DNA. • Isolation of RNA. Shipping at room temperature

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