DNA polymerase reagent kit Bst II Pro

diagnosticfor isothermal amplificationgenetic

Bst II Pro DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with Bst II DNA Polymerase Large Fragment (Vazyme #P702), Bst II Pro DNA Polymerase Large Fragment combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymerase activity at temperatures above 50°C. It displays superior amplification speed, specificity, salt tolerance and thermal stability. The reaction system can be prepared at room temperature. Features Robust performance: Good specificity, sensitivity, and yields Hot-start technology: Allows for room temperature reaction setup High inhibitor tolerance: Compatible with different crude lysates Optimized formulation: Stably detect 10 copies/rxn templates