Bst II DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with the previous generation, Bst II DNA Polymerase Large Fragment has superior amplification speed, dUTP tolerance, salt tolerance and thermal stability.
Features
Robust performance: High sensitivity and specificity; stably detect 10 copies/rxn templates
High inhibitor tolerance: Compatible with different crude lysates
Optimized formulation: Good extension speed