Solution reagent kit FastPure

tissuefor bacterial isolationfor cell isolation

This kit is suitable for extracting genomic DNA from ≤200 μl of fresh or frozen anticoagulated whole blood, <25 mg of animal tissues, <5 × 106 of cultured cells and <3 × 109 cultured bacterial samples. The kit is based on silica gel column purification technology that eliminates the need for extraction using phenol/chloroform organic solvents or time-consuming alcohol precipitation step. With this kit, RNA, proteins, lipids and other inhibitory impurities can be removed at the greatest extent. The DNA obtained can be directly used in PCR, qPCR, enzyme digestion and virus detection. Features High quality and good integrity: The extracted DNA has the characteristics of high yield, good purity and complete fragments Wide sample applicability: Suitable for direct extraction of DNA from fresh or frozen anticoagulated blood, cells, animal tissues and bacteria samples Simple and efficient: Extraction from animal tissue can be completed rapidly in 30 min Features Wide sample applicability Vazyme #DC112 was used for the DNA extraction and purification of the following 21 samples. And the final DNA product was detected by agarose gel electrophoresis. The elution volume was 100 µl, and 1% agarose gel electrophoresis was run with a sample volume of 160 - 180 ng. Results show that the kit is widely compatible with sample types and purifies high quality genomic DNA from different species. High DNA Yields and Purity Genomic DNA was extracted from HEK 293 cells, rat liver tissue, and Staphylococcus aureus with Vazyme #DC112 ("V" in the figures), products of Suppliers A, B, C, and D, and similar products of Vazyme. The obtained DNA was detected for concentration and purity.