DNA ligase reagent ClonExpress Ultra

for molecular biology

ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. The unique ligase-independent system of this kit significantly reduces the self-ligation background of the vector. Restriction endonuclease sites do not need to be considered. Highly optimized 2 × ClonExpress Mix (It contains enhanced recombinase Exnase) can significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure. Product Advantages Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%