Buffer solution reagent C311 series

DNA ligasefor PCRphosphate

5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt-end ligation and TA ligation. Buffer with optimized ligation enhancer makes the reaction more efficient and convenient. Ligation can be completed in 5 min at 25°C. The product can be used directly to transform many chemically competent cells. Features Compatible: Compatible with sticky ends, blunt ends, TA, linker or adapter ligation Fast: Ligation can be completed quickly in 5 minutes at 25°C Convenient: Reaction mixture can be used directly for bacterial transformation