Solution reagent kit EasyAna

ELISA test

EasyAna dsRNA (Modified) Quantitative Detection Kit (ELISA) 2.0 is developed based on a double-antibody sandwich ELISA platform to quantitatively detect the amount of dsRNA in the IVT product or synthesized mRNA solution. Features Easy-to-operate: 1.Pre-coated ELISA Plates can slash over 20 hours* of coating time and minimize the risk of inaccuracies caused by human handling. 2.All components for the experiment are fully equipped Nucleic Acid Antibody Screening Techniques: Antibodies compatible with different dRNA sequences Excellent Performance: High analytical sensitivity LOD < 10 pg/ml, IOQ = 46.87 pg/ml (LOQ up to 0.025 ppm if mRNA yield is 2 mg/ml| High accuracy Recovery between 80%-120% High precision CV < 15%, ensuring the repeatable data High specificity Specifically identify dsRNA of longer thon 40 bp; not interfered with by other forms of nucleic acids or IVT tool enzymes) T7 RNA polymerase, etc.) Performance Dilution accuracy To evaluate the consistency of the test results of samples gradiently diluted to different concentration. The coefficient variation (CV) between the recalculated concentration and the theoretical concentration after different multiples of dilution of 300 ng/ml dsRNA is less than 20%. High specificity We verified that the ssRNA, dsDNA, ssDNA, and the in vitro transcriptional enzymes used during mRNA preparation did not interfere with the detection system within the linear range of 0.046–1.5 ng/ml. Inter-lot consistency High, medium, and low-concentration samples were tested with 3 different lots, 10 times per lot, and the CV of lot-to-lot is ＜15%.