DNA polymerase reagent WizPure™

enzymefor RT-PCRfor cDNA synthesis

WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second-strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. Overview •5' to 3' exonuclease: No •3' to 5' exonuclease: No •3’ – A Overhang: No •Fragment Size: Up to 10 kb •Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease •200 units/μl Application •Gene synthesis •First-strand synthesis of cDNA •RT-PCR •Random priming reactions •Library construction •Probe labeling •mRNA 5’-end mapping Storage & Shipping Shipped on ice packs and/or dry ice