WizPure™ PCR 2X Master is an economical, highly efficient, and ready-to-use PCR premix that can amplify templates of up to 5 kb. The amplification products are compatible with TA cloning. WizPure™ PCR 2X Master yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR, and PCR cloning. WizPure™ PCR 2X Master is a highly processive 5’→3’ DNA polymerase that lacks 3’→5’ exonuclease activity and lacks a 3’→5’ proofreading function.
Overview
•Including loading dye for easy gel electrophoresis
•A-tail formation at 3’ ends of amplified duplex DNA
•Ready-to-use solution
•Easy reaction setup
•Contains loading dye for visual confirmation and quick electrophoresis
•Minimal optimization requirements
Application
•High through-put PCR
•Routine diagnostic PCR requiring high reproducibility
•DNA sequencing template preparation