Stain reagent Giemsa

for hematologyfor karyotyping

Intended Use: This kit is for staining blood cells and chromosomes. Principle: Blood Cell Staining: Giemsa Stain is a mixture of methylene blue and eosin. Methylene blue is alkaline, and eosin is acid. Because each cell and its components are different in chemical composition, their affinities to acid stain and alkaline stain of this kit vary significantly. Consequently, one can identify cells based on their staining color, shape and other physical characteristics. Methods: Blood cell staining: - 1. Dry the blood smear and fix in Methyl alcohol for 1~3 mins. - 2. Place the fixed blood smear in diluted Giemsa stain (solution A: solution B = 1:9) for 10~30 mins. (Use drop stain if specimens are not too many.) At lower room temperature in winter, incubate at 37°C. - 3. Take out the slide and rinse with water. Dry the slide for microscopic examination. Chromosome staining: - 1. Pretreat the specimen with trypsin. - 2. Dip the specimen into diluted Giemsa stain (solution A: solution B = 1:9) for 10 mins. - 3. Rinse with water and examine under a microscope after drying the slide. Specifications: Contents - Giemsa solution A: 1 vial × 100 ml - Giemsa solution B: 4 vials × 250 ml Expected Results: Blood cell staining: Erythrocyte appears vermeil. Leukocyte appears varying shades of blue to dark blue. Structure of nuclei is clear. Cytoplasmic granules are stained clearly and appear the specific color of each cell. - Product Name: Giemsa Stain - Series: Hematology Series - Release time: 2016-8-10 - Intended use: Staining blood cells and chromosomes - Principle: Mixture of methylene blue (alkaline) and eosin (acid) - Kit contents: Giemsa solution A (1 vial × 100 ml), Giemsa solution B (4 vials × 250 ml) - Application: Blood cell and chromosome staining