1. Laboratory
  2. Laboratory medicine
  3. Taq DNA polymerase reagent
  4. Yeasen Biotechnology (Shanghai) Co., Ltd

Solution reagent 2×Hieff™
dyeTaq DNA polymerasefor PCR

solution reagent
solution reagent
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Characteristics

Type
solution, Taq DNA polymerase, dye
Applications
for PCR, for electrophoresis
Other characteristics
hot start
Storage temperature

-25 °C, -15 °C
(-13 °F, 5 °F)

Description

2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×HieffTM PCR Master Mix simplifies PCR procedure and reduces contamination. Features Convenient, ready-to-use mix Thermostable—half-life is more than 40 min at 95°C Generates PCR products with 3'-dA overhangs Applications Routine PCR amplification of DNA fragments High throughput PCR DNA labeling Specifications Fidelity (vs. Taq) - 1 × Hot Start - No Overhang - 3'-A Polymerase - Taq DNA Polymerase Reaction Format - SuperMix or Master Mix Reaction Speed - Standard Product Type - PCR Master Mix (2×)

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