Antibody Hieff™

DNA polymerasemonoclonal antibodyfor molecular biology

Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody is a double blocking Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease after binding to Taq DNA Polymerase, which can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification caused by primer dimer at low temperatures. In addition, the product can effectively prevent probe degradation. Hieff™ Double-Block anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, through which the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibodies. Applications The concentration of this product is 5 mg/mL. 1 μL antibody could block the activity of 20-50 U Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase at room temperature for 1 hour (incubate at room temperature for 2 hours when volume is greater than 200 mL, Note: The specific activity of different Taq DNA Polymerase is variant, the blocking ratio needs to be adjusted appropriately to achieve that the blocking efficiency is better than 95%.