Magnetic bead-based reagent kit R210 series

for RNA extractionNGSfor RT-PCR

Easy Handling: Bypass chloroform, phase separation and precipitation steps. NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included). Non-Biased: Complete RNA recovery without miRNA loss. DESCRIPTION The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc. Specifications Equipment - Microcentrifuge, vortex, magstand Sample Inactivation - TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. Sample Source - Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). Size Range - Total RNA ≥ 17 nt Yield - 10 µg RNA (binding capacity), ≥ 30 µl (elution volume)