Solution reagent kit Quick-16S Plus

NGSfor DNA library preparation

Fast: 8x less hands-on time than conventional 16S library prep protocols. Easy: Premixed Plate minimizes hands-on time and handling errors. Simple Workflow: 100% Automation ready with only a single PCR step and no need for normalization. Just pool by equal volume! DESCRIPTION The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes. Amplicon Size - The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~388 bp. Equipment Needed (user provided) - Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates. Index Sequences - 10 bp indexes are listed in the MiSeq Sample Sheet Template provided. Sample Input - Purified microbial DNA (≤100 ng), free of PCR inhibitors. Sequencing Platform - Compatible with all Illumina® sequencing platforms using custom sequencing primers. We recommend the MiSeq® Reagent Kit v2 (300-cycle).