DNA polymerase reagent ZymoTaq

dyefor genotypingSNP

Hot-start polymerase for easy setup at room temperature. Robust amplification of DNA for genotyping, SNP, and HRM analysis. Strong fluorescent signal for real-time and quantitative PCR assays. DESCRIPTION ZymoTaq qPCR PreMix contains all the reagents needed to perform quantitative PCR and other molecular downstream analysis such as high-resolution melt (HRM) analysis of DNA methylation and other general real-time PCR assays. This premix features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treated DNA. It also includes an intense dsDNA-specific fluorescent dye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits (E2005, E2006, E2007) for low copy detection of DNA from human, bacteria, or fungi. Activity - 5' - 3' DNA polymerization Concentration - Reaction conditions at 1X (50 µl total volume) will contain 2 U of ZymoTaq DNA polymerase. Equipment - Real-time PCR can be performed on any instrument that has optical scanners that do not require passive reference dye. Unit Definition - One unit (U) enzyme of ZymoTaq DNA Polymerase is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C.