Agarose gel reagent kit Zymoclean

for PCRfor nucleic acids

Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels. Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA. Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc. DESCRIPTION The Zymoclean Large Fragment DNA Recovery Kit is a gel DNA extraction kit that provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes. TECHNICAL SPECIFICATIONS Applicable ForHigh-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applications Elution Volume≥ 10 µl EquipmentMicrocentrifuge PurityHigh-quality, purified DNA is especially well-suited for sequencing and ligation reactions Sample SourceAgarose gels containing large DNA fragments of interest Size Range50 bp to 200 kb TypeLarge DNA YieldUp to 10 µg DNA