ABScript II cDNA First Strand Synthesis Kit features two optimized mixes, ABScript II Enzyme Mix and ABScript II Reaction Mix. The enzyme mix combines ABScript II Reverse Transcriptase and RNase Inhibitor, and the reaction mix contains an optimized buffer. ABScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, which provides higher specificity and higher yield of cDNA.
The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.