Antibody UltraScence Femto Plus

antigenIgGfor Western blot

The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates. The mid femtogram to low femtogram detection of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. - No optimization required. Switching to the UltraScence Femto Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Femto, does not require optimization or protocol changes. - High degree of sensitivity and enhanced chemiluminescence duration. UltraScence Femto Plus Western Substrate enables an accurate mid femtogram to low femtogram detection of protein on the same immunoblot after a single exposure. - Optimized for use with PVDF and nitrocellulose membranes. - Compatible with Western Blotting Markers. - Optimized for film and CCD-based imaging. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer.