COVID-19 detection kit QP019T-0100

SARS-COV-2clinicalnasopharyngeal

In view of the joint global efforts of advancing collaborative research in diagnostics, therapeutics, and vaccination in the fight against the COVID-19 (SARS-CoV-2) pandemic, Bio-Helix has specifically developed the LifeDireX COVID-19 RT-qPCR Detection Kit Plus for human respiratory tract specimens. The kit is characterized by: (1) High specificity for the N target markers as recommended by WHO and US CDC; (2) Data obtained in less than 2 hours; and (3) Compatible with standard RT-qPCR machines (ABI 7500, Bio-Rad CFX96, QuantStudio’s 7 Flex). Application 》Gene Expression (mRNA) Analysis 》Copy Number Analysis 》SNP Genotype Analysis A study was performed to assess the performance of COVID-19 RT-qPCR Detection Kit Plus. A testing of 20 replicates at the tentative Limit of Detection (LoD) concentration was carried out with 50 copies/reaction at 95% detection rate. Samples were spiked SARS-CoV-2 RNA in pooled negative clinical nasopharyngeal swab matrix, then extracted with QIAamp Viral RNA Mini Kit and tested on QuantStudio™ 5 Real-Time PCR System. The synthetic SARS-CoV-2 RNA was obtained from Twist Biosciences (Assay Ready Control 14,EPI_ISL_710528). A study was performed to assess the clinical performance of COVID-19 RT-qPCR Detection Kit Plus. A testing of 20 replicates at low positive with 100 copies/reaction, 10 replicates at high positive with 2500 copies/reaction and 30 replicates were unspiked. Samples were spiked SARS-CoV-2 RNA in pooled negative clinical nasopharyngeal swab matrix, then extracted with QIAamp Viral RNA Mini Kit and tested on QuantStudio™ 5 Real-Time PCR System.