Solution reagent EvaGreen®

for microbiologyfor researchfor DNA quantification

Detection and quantification of DNA and cDNA targets. Profiling gene expression. Microbial detection. Viral load determination. DESCRIPTION: Titan HotTaq EvaGreen® qPCR Mix is optimized for real-time quantitative PCR assays. The mix includes Titan HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye (and ROX dye according to system requirements). Only template primers and water need to be added. Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Titan HotTaq EvaGreen® qPCR Mix is available with ROX or no ROX dye. Cycler compatibility: Titan HotTaq EvaGreen® qPCR Mix (ROX) can be used with all major qPCR cyclers requiring high ROX dye level, including ABI PRISM® 5700, 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block); Stratagene Mx3000P™, Mx3005P™ and Mx4000®. Titan HotTaq EvaGreen® qPCR Mix (no ROX) can be used with all major qPCR cyclers that do not require reference dye, including Bio-Rad iQ™ 5, Opticon™, Opticon™ 2; Chromo 4™, MiniOpticon, CFX96, CFX384; Corbett Rotor-Gene™ 3000, Rotor-Gene™ 6000; Roche LightCycler® 480 and Techne Quantica™. Composition: Titan HotTaq DNA polymerase 5x qPCR buffer E 12.5 mM MgCl2: 1 x PCR solution – 2.5 mM MgCl2 dNTPs, including dTTP to improve reaction sensitivity and efficiency EvaGreen®dye* ROX dye** or no ROX dye