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Solution reagent Titan HotTaq EvaGreen®
for researchfor microbiologyfor qPCR

solution reagent
solution reagent
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for research, for microbiology, for qPCR, for DNA quantification


Titan HotTaq EvaGreen® qPCR Mix APPLICATIONS: Detection and quantification of DNA and cDNA targets. Profiling gene expression. Microbial detection. Viral load determination. DESCRIPTION: Titan HotTaq EvaGreen® qPCR Mix is optimized for real-time quantitative PCR assays. The mix includes Titan HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye (and ROX dye according to system requirements). Only template primers and water need to be added. Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Titan HotTaq EvaGreen® qPCR Mix is available with ROX or no ROX dye. Cycler compatibility: Titan HotTaq EvaGreen® qPCR Mix (ROX) can be used with all major qPCR cyclers requiring high ROX dye level, including ABI PRISM® 5700, 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block); Stratagene Mx3000P™, Mx3005P™ and Mx4000®. Titan HotTaq EvaGreen® qPCR Mix (no ROX) can be used with all major qPCR cyclers that do not require reference dye, including Bio-Rad iQ™ 5, Opticon™, Opticon™ 2; Chromo 4™, MiniOpticon, CFX96, CFX384; Corbett Rotor-Gene™ 3000, Rotor-Gene™ 6000; Roche LightCycler® 480 and Techne Quantica™.
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.