Solution reagent Titan HotTaq Probe Capillary Mix

DNA polymerasefor researchfor DNA quantification

Detection and quantification of DNA and cDNA targets. Profiling gene expression. Microbial detection. Viral load determination. DESCRIPTION: Titan HotTaq Probe qPCR Capillary Mix is optimized for real-time quantitative PCR assays for qPCR cyclers with capillaries. The mix contains several components necessary to perform qPCR, including Titan HotTaq DNA polymerase. Only template, primers, probe and water needs to be added. Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C, preventing the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Cycler compatibility: Titan HotTaq Probe qPCR Capillary Mix can be used with LightCycler® 1.x and LightCycler® 2.0 (Roche Applied Sciences). Composition Titan HotTaq DNA polymerase 5x qPCR Buffer P 15 mM MgCl2: 1 x PCR solution – 3 mM MgCl2 dNTPs, including dTTP to improve reaction sensitivity and efficiency BSA