Bionano Prep Kits™ provide the critical reagents and protocols needed to extract and label high molecular weight (HMW) DNA for use on the Irys® and Saphyr™ genome mapping systems. Bionano kits are optimized for performing Bionano genome mapping applications on a variety of sample types.
HIGH MOLECULAR WEIGHT DNA EXTRACTION
Bionano genome mapping begins with the isolation of HMW DNA
Bionano sample kits are optimized for isolating and purifying HMW DNA in a process that is gentler than existing HMW DNA extraction methods. The resulting purified DNA, several megabases in length, is optimal for use with Bionano systems. Each Bionano Prep Kit allows you to perform 5-10 HMW DNA preps. Bionano kits and protocols enable the extraction of HMW DNA from a variety of sample types including soft or fibrous:
Bone Marrow Aspirate (BMA) – NEW!
LABELING REAGENTS DEVELOPED SPECIFICALLY FOR BIONANO SYSTEMS
Bionano labeling reagents are optimized for applications on Bionano genome mapping systems
Starting with HMW DNA purified using the appropriate Bionano prep kit, fluorescent labels are attached to specific sequence motifs. The result is uniquely identifiable genome-specific label patterns that enable de novo map assembly, anchoring sequencing contigs and discovery of structural variations starting at 500 bp.