Enzyme reagent kit

proteaseRNase inhibitorRNA Synthesis

T7 RNA polymerase a product purified after being synthesized by expressing the gene for bacteriophage T7 RNA polymerase in Escherichia coli. This allows to specifically bind onto the T7 phage promoter site and catalyze the RNA synthesis from the cloned DNA sequence located at the bottom of the T7 promoter site. ※This product is shipped in dry ice. Features and Benefits High Specificity Has high specificity to T7 phage promoter sequence allowing synthesis of desired RNA transcriptome. Versatility Conveniently synthesize the mRNA and sgRNA for such as for vaccine production and CRISPR-Cas9 researches. Reproducibility Manufactured under ISO9001 Quality management systems to produce each batch with unformed quality, allowing reproducible results. Application Synthesis of highly radiolabeled RNA probes Synthesis of precursors of siRNA Synthesis of precursors for RNA splicing reactions Synthesis of mRNA for in vitro translation Synthesis of sgRNA for gene targeting RNA structure, processing and catalysis studies Expression control via antisense RNA Specifications DNase activity No RNase activity No Protease activity No Components • 5X Reaction buffer with MgCl2: 200 mM Tris-HCl, 30 mM MgCl2, 10 mM spermidine, pH 8.0 • RNase Inhibitor: 100 ng/µl • 100 mM DTT ▶ Concentration 5,000 units (50 U/μl) ▶ Storage conditions 50% (v/v) glycerol containing 20 mM sodium phosphate, 100 mM KCl, 0.5 mM EDTA, 1 mM DTT, Stabilizers, pH 7.7 Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 60 minutes at 37℃