Antibiotics reagent P0017 series

G418for bacterial culturesfor cell culture

Overview
G-418 (Geneticin) sulfate is an aminoglycoside antibiotic used for selection of genetically modified prokaryotic and eukaryotic cells expressing G-418 resistance markers. Supplied as a powder for preparation of aqueous working solutions.

Applications

• Cell culture selection
• Stable cell line generation and maintenance

Solubility
Soluble in water at 50 mg/ml, yielding a clear to very lightly hazy colorless solution.

Recommended working concentrations
The optimal concentration for selection and maintenance must be determined for each cell line.

• Bacteria and algae: 5 µg/ml
• Mammalian cells: 400 µg/ml (selection); 200 µg/ml (maintenance)

Available volumes

• 1 g
• 5 g
• 10 g

Product details
Product code: P0017
State: Powder
Shipping: Room temperature or controlled temperature
Storage: +2/+8°C
Shelf life: 36 months
Sterility: Non-sterile

Technical specifications

• Active ingredient: G-418 (Geneticin) sulfate
• Form: Powder
• Solubility: 50 mg/ml in water (clear to very lightly hazy)
• Recommended concentrations: bacteria/algae 5 µg/ml; mammalian cells selection 400 µg/ml, maintenance 200 µg/ml
• Available packagings: 1 g, 5 g, 10 g (other packagings on request)
• Shipping: Room temperature or controlled temperature
• Storage conditions: +2/+8°C
• Shelf life: 36 months
• Sterility: Non-sterile