Solution reagent CTL-Wash™

reagent mediumfor cell cryopreservation

Specially formulated, nutrient-rich supplement for PBMC CTL-Wash™ supplement is a 10x serum-free formulation that should be added to RPMI-1640. CTL-Wash™ contains injection-grade water supplemented with amino acids, vitamins, inorganic salts, human serum albumin in a proprietary composition to provide a nutritionally complete and balanced environment for the PBMC for maximizing PBMC survival. Helps maintain full viability and functionality throughout washing of PBMC following isolation or thawing. The two to three washing steps required after the Ficoll® isolation or during thawing of PBMC and the time required for their counting typically adds up to 30-40 minutes During this time, the PBMC quality will deteriorate if kept in a nutritionally-deficient environment If uncharacterized serum is added, mitogenic or toxic effects can affect the cells Details CTL-Wash™ Supplement 10x is a buffered, serum-free solution containing injection-grade water supplemented with amino acids, vitamins, inorganic salts, and human serum albumin in a proprietary composition to provide a balanced environment for PBMC. CTL-Wash™ Supplement 10x is sold in convenient sterile 100ml units ready for dilution in 900ml sterile RPMI-1640. Slight fluctuations in pH might occur resulting in change of color – however, such changes do not affect the medium’s performance.