Solution reagent HOTSHOT

dyefor qPCRfor DNA analysis

Our PCR Master Mix is designed to amplify DNA irrespective of the source with the use of a thermocycler Use with or without dye or probes to allow maximum flexibility for qPCR or End Point PCR High Resolution Melting: HotShot Diamond has been used for many years with LCGreen Plus dye as a convenient and effective kit for HRM HotStart Ab mediated Taq to ensure specificity Use with gDNA, cDNA, and Viral A robust PCR Master Mix supplied as 5x1mL in a 2x concentrated form 500 Reactions at 20ul Can be supplied with ROX for ease of use Description Features & Benefits All of our PCR Master Mixes go through stringent QC/QA procedures to ensure batch to batch performance The Taq polymerase used in our PCR Master Mix is inactive until the PCR Master Mix has been incubated at 95C for five minutes thus ensuring greater specificity They contain everything you need for PCR except DNA and Primers We’ve optimised a starting MgCl2 concentration of 6mM and alongside our Taq polymerase we’ve added our proprietary PCR buffers to ensure that you get required results from the start They work with 3 step, 2 step and touchdown PCR protocols We supply our PCR Master Mix in a 2x concentration for convenience (for set up of both small & large volume reactions) They can be used for amplifying sequences with sizes below 100bp and up to at least 1.5kb High Resolution Melting HotShot Diamond PCR Master Mix works very well for High Resolution Melting with LCGreen Plus™ dye (BioFire Inc). LCGreen Plus dye has been shown to be the best dye for High Resolution Melting techniques.