Solution reagent SURESHOT

dyefor PCRfor DNA analysis

PCR Master Mix for amplification of DNA using PCR Exceptional amplification for everyday applications. Supplied as a ready to use 1x concentration in 5x1mL. Other formats available Please contact us for details Description Features & Benefits All of our PCR Master Mixes go through stringent QC/QA procedures to ensure batch to batch performance Our PCR Master Mix is designed to amplify DNA irrespective of the source with the use of a thermocycler The Taq polymerase used in our PCR Master Mix is inactive until the PCR Master Mix has been incubated at 95C for five minutes thus ensuring greater specificity They contain everything you need for PCR except DNA and Primers We’ve optimised a starting MgCl2 concentration of 6mM and alongside our Taq polymerase we’ve added our proprietary PCR buffers to ensure that you get required results from the start They work with 3 step, 2 step and touchdown PCR protocols We supply our PCR Master Mix in a 2x concentration for convenience (for set up of both small & large volume reactions) They can be used for amplifying sequences with sizes below 100bp and up to at least 1.5kb High Resolution Melting HotShot Diamond PCR Master Mix works very well for High Resolution Melting with LCGreen Plus™ dye (BioFire Inc). LCGreen Plus dye has been shown to be the best dye for High Resolution Melting techniques. Is our HotShot PCR Master Mix a mix for all the seasons? Perhaps, but we also offer the SureShot PCR Master mix for routine PCR aimed at gel electrophoresis. HotShot Sapphire PCR Master Mix for those multiplex assays that are going straight to gel. We’ve added an inert blue dye to our Sapphire for you.