Protein ladder BLUelf

for Western blot

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heats, dilute, and add reducing agent before loading. Features Broad range: 3.5-245 kDa (Tris-glycine-SDS running buffer) Ready-to-use: supplied in a loading buffer for direct loading on gels Easy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye Sharp bands Applications Monitoring of protein migration during SDS-PAGE. Monitoring of protein transfer onto membranes during Western blots. Sizing of proteins on SDS-PAGE and Western blots. Storage Buffer Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Quality Control The quality of the BLUelf Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.