Illumina DNA PCR-Free Prep offers a rapid and flexible workflow for preparing libraries for use in sensitive whole-genome sequencing applications, such as human whole-genome sequencing (WGS), de novo assembly of microbial genomes, and tumor–normal variant calling.
Reduces cost by eliminating pre- and post-library quantification steps
Simplify lab operations
The Illumina DNA PCR-Free Prep workflow supports a broad DNA input range, multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots.
Provides highly uniform coverage across repetitive or uneven genome regions
Obtain reliable results
On-bead tagmentation chemistry combined with PCR-free library preparation eliminates PCR-induced bias and diminishes opportunities for error, providing superior and even coverage across high-GC or -AT regions. Learn more about on-bead tagmentation.
Delivers fast, automation-compatible workflow in 90 min
Access flexible throughput
The Illumina DNA/RNA UD Indexes Sets offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell.
Illumina DNA Prep product line
Find fast, optimized sequencing library preparation solutions for use in a wide range of applications.
Specifications
Assay time - ~1.5 hr
Automation capability - Liquid handling robot(s)
Automation details - Explore available automation methods
Description - Prepare sequencing libraries for sensitive applications using a unique
combination of on-bead tagmentation with PCR-free chemistry which simultaneously eliminates PCR-induced bias and reduces total library prep time to 90 minutes.