Solution reagent kit AmpliSeq™

blood samplefor PCRfor nucleic acids

The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair. Key features Relevant Gene Content Target 161 unique cancer-associated genes Fast, Streamlined Workflow Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples Accurate Data Detect somatic mutations down to 5% frequency using local or cloud-based analysis The Comprehensive Panel v3 is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis. The panel content spans hotspot regions, full-length genes, copy number genes, and inter- and intragenic gene fusions. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. Specifications Assay time - 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) Cancer type - Solid tumor Content specifications - DNA and RNA targets for 161 oncogenes Description - Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. Hands-on time - <1.5 hr Input quantity - 1–100 ng (10 ng recommended per pool) Multiplexing - 96 dual index combinations Variant class - Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs)