Research test kit 3420-2HPT-2

cellEliSpot

One-step detection – our most convenient ELISpot assay. Detection mAb - 7-B6-1, HRP Substrate - ELISpot substrate: TMB for HRP Plate - Pre-coated ELISpot plate, clear MSIP (mAb 1-D1K) Positive control - Anti-CD3 mAb (CD3-2) Intended use This ELISpot Pro kit is intended for the enumeration of cells secreting human IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures. Product - ELISpot Pro: Human IFN-γ (HRP) Application - ELISpot Analyte - IFN-γ Reactivity - Human Specificity This kit is based on a matched pair of mAbs specific for human IFN-γ. Performance PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IFN-γ was analyzed by ELISpot. PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of the CEF peptide pool (available from Mabtech, code 3616-1) and the number of cells secreting IFN-γ was analyzed by ELISpot. Analyte description Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. Alternative names - Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69