Research test kit 3421M-2H

veterinaryELISA

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice. Components Capture mAb - MT126L Detection mAb - 7-B6-1, biotin Enzyme conjugate - Streptavidin-HRP Intended use This ELISpot Flex kit is intended for the enumeration of cells secreting monkey IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures. Product details Application - ELISpot Analyte - IFN-γ Reactivity - Non-human primates Specificity - This kit is based on a matched pair of mAbs enabling specific detection of IFN-γ from non-human primates (NHP). Cross-reactivity - The mAbs react with human IFN-γ and cross-react with IFN-γ from NHP. For more information please check the Cross-reactivity guide section below. Performance PBMC from cynomolgus macaque were incubated overnight with recombinant rhesus CMV protein Rhpp65 (250,000 cells/well) or medium only (250,000 cells/well) and the number of cells secreting IFN-γ was analyzed by ELISpot. PBMC from cynomolgus macaque were incubated overnight with anti-CD3 mAb (50,000 cells/well) or medium only (250,000 cells/well) and the number of cells secreting IFN-γ was analyzed by ELISpot. Non-human primate cross-reactivity The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP). Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses. Cross-reactivity Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.