Computer-controlled patch-clamp system Port-a-Patch

whole-cellminiature

The Port-a-Patch is a miniaturized patch clamp system supporting giga-seal recordings from one cell at a time. It offers fast and easy access to high quality patch clamp data with only minimal training. Not only a powerful research tool but also ideal for educational purposes and quick tests of cells and ion channels. The benefits the Port-a-Patch offers include: External & internal perfusion Temperature control Voltage & current clamp Whole-cell & perforated patch clamp Chips produced in-house, various resistances available (customizable on request) Rseries compensation Unlimited compound applications Voltage and ligand-gated ion channels High success rates with cell lines; use of primary cells and stem cells also possible Easy to use, minimal training required Adjustment of parameters during experiments for fast and efficient assay development Using the Port-a-Patch is straightforward and easy – the user simply adds solutions and cells onto the disposable recording chip, where a cell is automatically captured and sealed by suction using a computer controlled pump. A microscope or micro-manipulator are not required. Add-Ons such as the Internal and External Perfusion and Temperature Control, as well as the ability to conduct current clamp and bilayer experiments, broaden the range of possible experiments for scientific research immensely. The Port-a-Patch system is comprised of the Port-a-Patch recording unit, a Suction Control unit, a HEKA amplifier and a computer. If an amplifier is already available, this can be integrated with the Port-a-Patch system in most cases.