Fully automated sample preparation system SURFE²R 96SE

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Just like the SURFE²R N1, the SURFE²R 96SE employs SSM-based electrophysiology to resolve small currents of transporters and pumps, but elevates the throughput to the next level by parallelization of 96 measurements. The transporter samples are added to gold coated sensors in a standard 96 well-plate format. To further reduce the user's workload, all sensor coating steps can be done by the SURFE²R 96SE itself. Any substrate can be added to activate the transporter. The resulting transport or binding currents will be detected and analyzed accordingly. The whole process, from sample preparation and measurement to data analysis, is fully automated. • Recording of 96 wells in parallel using a 96-channel low-noise amplifier • 10,000 data points per day • Integration into the small footprint pipetting platform CyBio® Felix • Automatization of experimental workflows including sample preparation • Automated data analysis and export, e.g. determination of EC50 • 8 positions for different solutions (1 well to 96 well) • > 100 targets validated, 100 peer reviewed papers using SSM-based electrophysiology • Transporters, pumps and ligand gated channels • Measure even electroneutral exchangers and sugar binding • Use purified membranes and vesicles from cells or proteoliposomes • Label-free electrical measurements • Requires only 0.1 – 1 µg protein per sensor. This is sufficient for up to 100 experiments • Real-time data with high time resolution of 5 ms, not single point read-out • High signal amplification compared to patch-clamp • Fast binding kinetics can be resolved • EC50, IC50, rate constants, comparison of transporter variants,...