CDNA synthesis reagent kit MB1250 series

reverse transcriptaseRT-qPCRenzymatic

NZY First-Strand cDNA Synthesis Kit is a convenient and reliable system that includes all the necessary components to synthesize first-strand cDNA. The kit allows reaction at high temperatures, which are benefic when starting from difficult and complex RNA. Details NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA. Components – NZYRT Enzyme Mix – NZYRT 2x Master Mix – NZY RNase H (E. coli) – DEPC-treated H2O More Information Shipping Conditions Dry Ice Features Dual-primer: oligo(dT) + random hexamers, Fast, High yield, Thermostable Reaction Temperature 50 °C