DNTP solution reagent kit Clara™ HRM

Taq DNA polymerasedyefor qPCR

SNP Genotyping Methylation status analysis Gene scanning studies This qPCR mix delivers maximum specificity to your high resolution melt (HRM) curve analyses. Accurately detect genetic mutations, quickly identify genotypes based on SNPs, or calculate percent methylation of a target region with HRM analysis. Clara™ HRM Mix comprises our unique, ultra-pure, PCRBIO Taq DNA Polymerase in a blend containing dNTPs and MgCl2. Powered by our third-generation, DNA-intercalating, SyGreen 2 dye for greatly reduced PCR inhibition, this new generation high-resolution melting qPCR mastermix offers superior performance for accurate SNP discrimination and quantification of methylation differences. Features Accurate distinction of SNP classes I-IV Quantify methylation of target sequences Super-sensitive product melt curves for distinct allele profiles Compatible with all HRM-suitable real-time instruments Powered by PCRBIO Taq DNA polymerase What is HRM? High Resolution Melt (HRM) curve analysis is a powerful technique used for the analysis of mutations, polymorphisms and epigenetic differences in double stranded DNA samples. This method relies on analysis of the post amplification denaturation of qPCR targets in combination with third generation DNA intercalating dyes with reduced polymerase inhibition (e.g. SyGreen 2, EvaGreen®). HRM analysis exploits the differences in melt curve shapes and DNA melting temperature to discriminate sequence variations between samples. Usually, in a melt curve analysis run after a standard dye-based qPCR, fluorescence data is acquired every 0.2-0.5 °C,whereas in HRM analysis data is acquired at a much higher density, every 0.1 °C,