Reverse transcriptase reagent kit qPCRBIO SyGreen 1-Step

dyeRNase inhibitorfor real-time PCR

Thermostable reverse transcription Sensitive Early Ct Select from qPCRBIO SyGreen 1-Step Detect for the detection of extremely low copy number targets, or qPCRBIO SyGreen 1-Step Go for rapid and accurate results from high RNA template concentrations. Features Thermostable reverse transcriptase 45°C to 55°C Advanced RNase inhibitor Non-PCR inhibiting intercalating dye Rapid extension rate for early Ct values Market-leading sensitivity Increased limit of detection Antibody-mediated hot start PCR Compatible on all standard and fast cycling real-time PCR platforms More Information qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube. qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template concentrations. The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity.