Reverse transcriptase reagent kit UltraScript™

for cDNA synthesisfor nucleic acids

The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimised blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Features Unbiased representation of 5’ and 3’ mRNA transcript ends Reduced RNase H activity Thermostable reverse transcriptase 45 °C to 55 °C Advanced RNase inhibitor High cDNA yields from as little as 4 pg total RNA Accurate reverse transcription of GC-rich templates Sensitive detection of low copy number transcripts Advanced buffer chemistry including Mg and dNTPs Both premixed or separate oligo cDNA synthesis kits available More Information UltraScript™ Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase (RTase). The reaction temperature can be increased up to 55 °C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript™ Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate. The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield cDNA from as little as 4 pg RNA.