UltraScript 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation of the most challenging RNA sample types.
This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full length cDNA product.
Highly thermostable reverse transcriptase 55°C to 65°C and above
Advanced RNase inhibitor
High cDNA yields from as little as 20pg total RNA
Accurate reverse transcription of GC-rich and highly structured transcripts
High sensitivity for challenging templates
Reduced RNase H activity
Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. The enzyme remains partially active even up to 90°C and is designed for efficient reverse transcription of the most difficult RNA templates, including GC-rich and highly structured transcripts.
UltraScript 2.0 RTase is designed for efficient cDNA synthesis from a broad range of RNA concentrations. UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase.