Solution reagent IC204

fixative solutionformalinfor histology

Overview
Neutral Buffered Formalin 10% is a ready-to-use aqueous formaldehyde solution (corresponding to 4% formaldehyde) supplied by Pro-Eko Srl for professional laboratory, diagnostic and technical applications. The product is formulated to provide stable, reproducible fixation under neutral pH conditions and is intended for use by trained personnel in regulated workflows.

Chemical information

• IUPAC name: methanal
• Chemical formula: CH2O
• CAS number: 50-00-0
• Formaldehyde content in solution: 4% (formalin 10%)

Mechanism of action
Fixation is achieved through formation of methylene bridges by the active form of formaldehyde (methylene glycol), producing intra- and intermolecular cross-links. These covalent bonds involve functional groups of biological macromolecules (amino acid residues, carbohydrate components), stabilizing tissue architecture and cellular components for downstream analyses.

Technical documentation available

• Technical Data Sheet (TDS)
• Safety Data Sheet (SDS) compliant with CLP
• Certificate of Analysis (CoA), where applicable

Usage and compliance
The solution is supplied ready-to-use, stabilized with a phosphate buffer and adjusted to neutral pH. Formulation prepared according to established histological criteria (Lillie method). Regulatory references include REACH and CLP where applicable. Sale and use are restricted to professional customers and trained operators in structured professional contexts.

Professional application areas

• Histology and anatomic pathology laboratories
• Diagnostic and research laboratories
• Scientific and technical facilities requiring chemical fixation of tissues
• Preparation of biological samples for subsequent analyses

Technical specifications

• Commercial name: Neutral Buffered Formalin 10%
• Formulation: Lillie method; phosphate buffer
• Nominal pH: 7.00
• Nominal osmolarity: approx. 1500 mOsm
• Formaldehyde content in solution: 4% (formalin 10%)
• IUPAC name: methanal
• Chemical formula: CH2O
• CAS number: 50-00-0
• Unit volume: 8 L
• Unit weight: 9 kg
• Internal product code: IC204
• Intended use: professional B2B use only