For immediate stabilization of DNA, RNA, and protein in tissues
No need for liquid nitrogen or dry ice
Immediate, convenient stabilization of harvested tissues
Long-term storage of tissues for later analysis
Standardized sample preparation for systems biology
Allprotect Tissue Reagent provides immediate stabilization of DNA, RNA, and protein in tissue samples at room temperature. This preserves the in vivo profile of DNA, RNA, and proteins, allowing reliable downstream analysis. Stabilized tissues can be transported at 15–25°C for up to 7 days, or stored at 2–8°C for up to 12 months. For longer storage, stabilized tissues can be archived at –20°C or –80°C.
Immediate stabilization of harvested tissues in Allprotect Tissue Reagent preserves the in vivo profile of DNA, RNA, and proteins. This enables reliable downstream analysis even with tissues stored at room temperature (15–25°C) for up to 7 days (see figures "Intact protein for western blotting", "Prevention of protein degradation", and "Preservation of protein activity"), Stabilization is especially critical for RNA, since transcript levels change rapidly after tissue harvesting (see figures "Prevention of gene induction" and "Prevention of RNA degradation"). Agilent 2100 Bioanalyzer analysis of RNA from Allprotect stabilized tissues gives high RIN values, indicating intact RNA. Allprotect Tissue Reagent is also able to stabilize molecules of increasing interest such as miRNA, methylated genomic DNA, and phosphorylated protein.
Freshly harvested tissue is submerged in Allprotect Tissue Reagent to immediately preserve the in vivo profile of DNA, RNA, and protein.