PCR reagent kit Investigator Triplex AFS QS
for genotypingfor forensics

PCR reagent kit
PCR reagent kit
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Characteristics

Applications
for PCR, for genotyping, for forensics

Description

For multiplex amplification of FGA (FIBRA), SE33 (ACTBP2), and Amelogenin Simultaneous amplification of 3 markers Includes an internal control for PCR efficiency Highly suited for low-copy and degraded DNA samples Highly sensitive and inhibitor resistant The Investigator Triplex AFS QS Kit is for forensic stain pre-selection, which serves as a starting point for genotyping strategies. The genetic analysis is calibrated with DS-30 matrix. Product Details Performance The Investigator Triplex AFS QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers. The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.1–1.0 ng DNA, and reliable results with <0.1 ng DNA. Principle The Investigator Triplex AFS QS Kit enables the simultaneous amplification of the two polymorphic STR loci FGA (FIBRA) and SE33 (ACTBP2), and the gender-specific Amelogenin. The kit includes optimized primers and reagents, and the relevant DNA control and allelic ladder. The QS in the name stands for Quality Sensor. This special feature is an internal PCR control that provides information on the presence of PCR inhibitors and the efficiency of the PCR. Procedure FGA (FIBRA), SE33 (ACTBP2), and Amelogenin are simultaneously amplified in a single PCR and labeled with 6-FAM. The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software.

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